The numerous positive effects of cycling on health, the environment, accessibility and sustainable development make the bicycle a more convenient means of transport at short and medium distances. On the other side, cyclists are vulnerable road users, as they are at high risk in traffic, which in some areas significantly affects the further development of cycling. In this paper were analyzed traffic accidents and consequences of accidents with cyclists that have occurred in the period from 2001 to 2018. in Serbia. An analysis of the number and structure of traffic accidents, spatial and temporal distribution, public risk and other characteristics of the cyclists fatality has been carried out. The aim of this research is to analyze the situation and tendencies of the safety of cyclist traffic in Serbia. The task of the research is to define and describe the general characteristics of the problem of the cyclists fatality in traffic. So the results of the paper give an answer when, where and how cyclists in Serbia are injured or killed.